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KMID : 0613820080180020241
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Journal of Life Science
2008 Volume.18 No. 2 p.241 ~ p.248
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Study on Anti-Helicobacter pylori Antibody of Sparated Antigen from H. pylori
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Park Chang-Ho
Bae Man-Jong
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Abstract
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This study has been carried out to secretion antibodies for the purpose of preventing the infection of Helicobacter pylori and using them as a supplement for treatment. This experiments have been separated antigens from H. pylori and observed into antibody production and the agglutination of H. pylori for the separated antigens. As major antigenic proteins separated from H. pylori, the following could be verified: 12 kinds of band for whole cell (WC), seven kinds of band for outer membrane protein (OMP), three kinds of band for crude urease, and one kind of band for lipopolysaccharide (LPS). The IgG anti-H. pylori antibody of separated antigens showed for we (L), for OMP, and for crude urease, at the same antigen concentration of , which showed the most at the crude urease. And it turned out that the IgA antibodies were generated with for WC (L), for OMP, and for crude urease, which demonstrated the most for WC (L) antigens. As a result of verifying the immunogenecity of antigenic protein through the Western blotting, major antigenic substances could be confirmed as follows: 10 kinds for WC, six kinds for OMP and three kinds for crude urease. The agglutination values on the H. pylori of the antibody were at the antigen serums of anti-WC (H), anti-WC (L), anti-OMP and anti-crude urease, respectively, which indicated the highest for the antigen serum of anti-crude urease. The urease activation-inhibiting absorbance of antigen serum created by each antigen was for WC (H), for WC (L), for OMP, and for urease, demonstrating a significant inhibiting effect, compared with of the control group.
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KEYWORD
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Helicobacter pylori, anti-H. pylori antibody, whole cell, outer membrane protein, lipopolysaccharide, urease
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